LED Lighting Source for Optical Microscope

 Product Description Key FeaturesExceeds the brightness of 100W Mercury LampCool lightInstant ON/OFF. No warm-up or cool-down requiredAdjust light outputEasy installation Product Parameters Wavelength Range: 350-780nm  External Power Supply: Universal input 110-240V, 50/60HzRelated Fluorescence: DAPI, GFP/FITC, Texas Red, Cy3Sample LED FWHM: 454/24.8nm  523/36nm  368/16nm  Power Consumption: 40WDimensions HEAD: 142×95×85mm (L×W×H)Dimensions TOUCH PAD: 130×126×20mm (L×W×H)LED ON/OFF Response Time: 1msI/O Connector: PS/2Control method: RS-232LED Life Time: 30000 hours                         BANDSBANDSCHANNELPOWER(mW)DAPIV330-38035FITCHB450-49065TRITCG510-55050 Installation Instructions All mercury arc lamps used a common heat absorbing filter from Schott…the KG1. Further, all Ploem illuminators (still today) used a RED absorbing filter, again from Schott, the BG-38 blue glass. These two filters are normally removed from the light path when one is working in the near UV range using filter block 400nm.I have measured the temperature of the illumination beam of the HBO 100W bulb using an analog "meat thermometer" with probe tip. I have measured the temperature of the collimated beam immediately after the KG1 heat filter and recorded temperatures in the range from 85-95F depending on placement position. I then measured the temperature down on the stage of the microscope with both the BG-38 and filter cube in position (no objective lens).Filters cubes used were the H (Blue light 510 block) and N(Green light 580 block). I found that the temperature range on the surface of the microscope stage where the slide would be placed was only a few degrees above room temperature. The KG1 and BG38 filters along with the combined exciter filter and emission (barrier) filters and filter blocks (dichroic beam splitters) were doing their job nicely. One problem encountered by those doing epifluorescence microscopy using the arc lamp is "quenching" of the fluorescent dye (fluorochrome). "Fading" of the emitted light during exposure became somewhat of a problem. Certain "antifade" tricks applied during sample prep became a useful solution to some extent. Certainly the advantage of using LED as an alternative to mercury arc lamps is now realized. One turns on the LED (optimized for the four dichroic blocks 400, 455, 510 and 580nm) and then simply reduces intensity as you would with a halogen bulb. LED illumination is not as "cool" as you would think. The same heat and red absorbing filters are called for. "Fading" (and temperature) is then reduced by adjusting the intensity of the LED. No warm up or shutdown problems with LED. No shutters are needed in the light path. The LED can easily be controlled via connection to your computer. LED illumination lasts 20-50,000 hours. Mercury arc bulbs last about 200 hours and cost about $200.00 each. Do the math and accept the performance enhancements found with modern LED illumination. We no longer use silver halide for documentation in microscopy….we are in a digital world now and the LED promises to be the most efficient light source for optical light microscopyfluorescence studies. Contact FSM with any questions. We have the best alternative LED lighting now for taking the place of mercury arc illumination. Company Profile With 6 years working, we succesfully get a good LED illuminator for microscope. It provides stable performance and over 20000 hours lifetime. Max 9 channels in a device. Detailed Photos