Wix-Fastblot Fast Semi-Dry Blot System

Basic Info.Model NO.WIX-fastBLOTWarranty1 YearCustomizedCustomizedStructureDesktopMaterialPlasticCertificationCEApplicationLabTypeWestern BlotOEMYesODMYesBlotFast Semi-DryTimeMax 7 MinsTrademarkWIXTransport PackageCarton/PalletOriginChinaProduct DescriptionWIX-fastBLOT Fast Semi-dry Blot System  Product Description1. Working condition1.1 Temperature: 5ºC~40ºC; Relative moisture: 20%~80%2. Technical requirement2.1*Blot throughout: 4 small gels or 2 medium gels; 2 blot cassettes, one of the two cassettes can be used for operation or two cassettes can be used respectively in the right orders. Two independent blot programme can be used at the same time. The blot area is 200*100mm*2.2.2 The world uniquely design for high efficient heat dissipation channel, is to release the heat during the operation and ensure the reliability of the lab in the low temperature.2.3 Easy operation: optional ready-to-use blot consumable package, no need to prepare buffer and membrane;2.5  *Blot speed: blotting 2 small gels within 3 minutes, blotting 4 small gels or 2 medium gels within 7 minutes.2.5  *Power: Built-in integrated power supply, voltage 0-30V (1V adjustable) current (0-3A) (0.1 adjustable).2.6 Customer interface, 5-inch capacitive touch screen, sensitive touching screen, convenient operation. Automatic programme running; The preset programme guides the lab design and modifies the programme manually on the basic of the needs (9 groups of data are available for recorded automatic storage and customer-decisive storage).2.7 Electrode Design: Buckle locking mode with plate electrode design ensure uniformity of pressure and field strength.2.8  Application: Being compatible with traditional lab reagent and consumable.2.9 Dimension (mm): 290x218x2003.0 Weight: 3.8kg3. Configuration requirement1 set of Blot System (including built-in power supply and 2 pieces of blot cassettes)Detailed Photos CertificationsPackaging & ShippingInstallation InstructionsExperiment preparationWix-fastBLOT Fast Semi-dry Blot System, fast transfer filter papers (5 sheets as 1 unit), gel, NC membrane or PVDF membrane (need to be activated with methanol before transfer), and roller. Experiment StepsPlace 1 unit of fast transfer filter papers (including 5 sheets of fast transfer filter papers) in the center of the cassette base. If transfer 1 mini gel, place the gel sandwich in the center of the cassette base, if transfer 2 mini gels, place 2 gels at two sides of the cassette base.Blotting buffer: Methanol: deionized water = 1:1:3 (V/V) ratio for diluting blotting buffer. Immerse fast transfer filter papers with blotting buffer until it is completely wetted (about 15 ml).NC or PVDF membrane is placed on top of the fast transfer filter papers, and use roller to remove any air bubbles.Carefully align the gel on the membrane. If necessary, gently use roller to remove air bubbles between gel and membrane.Place the other unit fast transfer filter papers (including 5 sheets of fast transfer filter papers) on top of the gel, immerse gel switch with blotting buffer to make it completely wetted (about 15 ml), use roller to remove any air bubbles.Put cassette lid on the base, ensure the red side of the lid and handle of the cassette base be aligned vertically, carefully press vertically and check to ensure the electrical contacts fit closely into slots in the base.Slide cassette into one the of fast blot bays until it makes contact with the magnetic interlock in the back of the instrument tub and you hear a click.Set transfer conditions according to below data. WIX-fastBLOT has two modes, one is A=B, set protocols of the two cassettes are the same, the other is A≠B, set protocols of the two cassettes are different.Molecular weight10-90kDa90-190kDa>190kDa1 mini gel per cassetteconstant current 1.3A 10 minconstant current 1.3A15 minconstant current 1.3A17 min2 mini gels per cassetteconstant current 2.6A 13 minconstant current 2.6A17 minconstant current 2.6A19 minTake out gel after blotting.Caution: Put cassette lid on the base, carefully press vertically and check to ensure the electrical contacts fit closely into slots in the base.If only 2 mini gels need to be transferred, A=B mode is recommended, 1 gel per cassette, under such conditions, generated heat by each cassette is a little so that to achieve a better experiment result. Put NC or PVDF membrane into block buffer immediately after it is taking out from the cassette, in order to avoid dried membrane leading by transfer liquid volatilization.If transfer time is longer that much heat generated, put NC or PVDF membrane into bock buffer immediately after it is taking out from the cassette, to avoid dried membrane.Company ProfileWIX TECHNOLOGY BEIJING CO., LTD (WIX for short) was established in 2015. It is a technology-based private manufacturing enterprise integrating product development, manufacturing, marketing, and technology consulting. WIX mainly develops and produces instruments in the field of life scienc